P-198. Discontinuous Percoll gradient preparation for donor insemination: determinants for success
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چکیده
منابع مشابه
Discontinuous Percoll gradient preparation for donor insemination: determinants for success.
The use of cryopreserved donor spermatozoa for insemination has become necessary to decrease the risk from sexually transmitted infectious diseases. Lower fecundity rates have been reported with this practice. Efforts have been applied to increase success, including identification of those sperm characteristics which correlate with increased fecundity. Data from in-vitro fertilization have reve...
متن کاملOptimizing success with donor insemination.
Artificial insemination with donor sperm yields pregnancy rates similar to the general fertile population with the woman's age being the best predictor for success. This article reviews the indications for donor insemination and the current American Society for Reproductive Medicine guidelines for screening both the donors and recipients. For most women, timing the insemination the day after de...
متن کاملPreparation of synaptoneurosomes from mouse cortex using a discontinuous percoll-sucrose density gradient.
Synaptoneurosomes (SNs) are obtained after homogenization and fractionation of mouse brain cortex. They are resealed vesicles or isolated terminals that break away from axon terminals when the cortical tissue is homogenized. The SNs retain pre- and postsynaptic characteristics, which makes them useful in the study of synaptic transmission. They retain the molecular machinery used in neuronal si...
متن کاملComparison of the coverslip and the discontinuous Percoll density gradient methods of enucleation of mouse cells.
Two methods of enucleation of LB 10 cells, a subline of mouse L cells, were used: the method of enucleation of cells growing in monolayers, and the newly improved method of enucleation in discontinuous Percoll gradients. The second method was more effective and, as shown by incorporation of 3H-lysine, protein synthesis in cytoplasts was prolonged twice when compared with that in cytoplasts obta...
متن کاملSeparation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient.
Cryptosporidium oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by density gradient centrifugation. This two-step method yielded highly concentrated oocysts largely free of bacteria and fecal debris.
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1997
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/12.suppl_2.212-a